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KMID : 0363420160300020096
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Journal of Korean Oriental Pediatrics
2016 Volume.30 No. 2 p.96 ~ p.106
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The Effects on Anti-inflammatory Action in HaCaT Cells and Inhibiting Sebum Secretion in SEB-1 Cells by Gleditsiae Fructus Extract
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Koo Eun-Jin
Han Jae-Kyung
Kim Yun-Hee
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Abstract
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Objectives: The purpose of this study is to investigate the effects of Gleditsiae Fructus 70% EtOH extract (JS_E) on
anti-inflammatory action in HaCaT cells (A spontaneously immortalized human keratinocyte cell line) and inhibiting
triglyceride genesis in SEB-1 cells (Immortalized human sebocyte).
Methods: The anti-inflammatory effect of JS_E was analyzed by enzyme-linked immunosorbent assays (ELISA) which
measured levels of IP-10, RANTES and MDC in HaCaT cells. Also the effect on secretion of sebum of JS_E was analyzed by TG-S kit which measured the quantity of triglyceride in SEB-1 cells.
Results: JS_E inhibited IP-10, RANTES and MDC expression in a dose dependent manner. IP-10 expression was inhibited significantly in comparison to TNF-α and IFN-γ recombination (TI) control group at concentration of JS_E 200 ㎍/㎖ and RANTES and MDC expressions were inhibited significantly at concentration of JS_E 100, 200 ㎍/㎖. JS_E also inhibited triglyceride secretion of SEB-1 cells significantly in comparison to the control group in a dose dependent manner.
Conclusions: This study shows that JS_E has the effects of anti-inflammatory action and inhibiting sebum secretion. According to these results, JS_E can be used for treating skin diseases such as acne and dermatitis caused by inflammation and excessive secretion of sebum by controlling the activity of the HaCaT and SEB-1 cells.
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KEYWORD
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Gleditsiae fructus, Acne, Inflammation, Sebum, HaCaT cells, SEB-1 cells, IP-10, RANTES, MDC, Triglyceride
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